Seroprevalence of avian influenza virus and its relationship with increased mortality and decreasedegg production

Abstract
To evaluate the occurrence of avian influenza viruses (AIV) infections in southern Pakistan, an enzyme-linkedimmunosorbent assay was initially used to screen sera from broiler, layer and broiler breeder flocks for thepresence of antibodies to type A influenza viruses. Data from this survey showed high levels of AIV antibodies,indicating unrecognized AIV infection occurring in these flocks. Based on this information a secondinvestigation was undertaken in selected broiler-breeder, broiler and layer flocks. In this investigation, nineH9N2 AIV isolates were recovered. Serological data from this investigation indicated that chickens in flockswith a previous history of respiratory tract infection and some without overt clinical respiratory signs hadseroconverted to H9N2 AIV.

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