Glucose reduces both Rb+ influx and efflux in pancreatic islet cells
- 5 May 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 200 (1) , 67-70
- https://doi.org/10.1016/0014-5793(86)80512-9
Abstract
Microdissected, β-cell-rich pancreatic islets from ob/ob mice were used in studies of 86Rb+ transport. D-Glucose (20 mM) induced a biphasic reduction in 86R+ efflux. The reduction stabilized within 10 min at 34% of the efflux rate at zero glucose. The initial 86Rb+ uptake (5 min) was dose-dependently reduced by ouabain with maximum inhibition at 1 mM. D-Glucose (20 mM) did not affect the ouabain-sensitive 86Rb+ influx but markedly reduced (48%) the ouabain-resistant isotope influx. The results suggest that D-glucose does not affect the Na+/K+ pump in pancreatic β-cells and that the glucose-sensitive K+-transporting modalities (K+ channels) in the β-cells can mediate both inward and outward K+ flux.Keywords
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