Highly efficient gene transfer into cord blood nonobese diabetic/severe combined immunodeficiency repopulating cells by oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD114) envelope protein
Open Access
- 15 August 2000
- journal article
- Published by American Society of Hematology in Blood
- Vol. 96 (4) , 1206-1214
- https://doi.org/10.1182/blood.v96.4.1206
Abstract
Limited expression of the amphotropic envelope receptor is a recognized barrier to efficient oncoretroviral vector–mediated gene transfer. Human hematopoietic cell lines and cord blood–derived CD34+ and CD34+, CD38− cell populations and the progenitors contained therein were transduced far more efficiently with oncoretroviral particles pseudotyped with the envelope protein of feline endogenous virus (RD114) than with conventional amphotropic vector particles. Similarly, human repopulating cells from umbilical cord blood capable of establishing hematopoiesis in immunodeficient mice were efficiently transduced with RD114-pseudotyped particles, whereas amphotropic particles were ineffective at introducing the proviral genome. After only a single exposure of CD34+ cord blood cells to RD114-pseudotyped particles, all engrafted nonobese diabetic/severe combined immunodeficiency mice (15 of 15) contained genetically modified human bone marrow cells. Human cells that were positive for enhanced green fluorescent protein represented as much as 90% of the graft. The use of RD114-pseudotyped vectors may be advantageous for therapeutic gene transfer into hematopoietic stem cells.Keywords
This publication has 68 references indexed in Scilit:
- A Defined Window for Efficient Gene Marking of Severe Combined Immunodeficient-Repopulating Cells Using a Gibbon Ape Leukemia Virus-Pseudotyped Retroviral VectorHuman Gene Therapy, 2000
- Human Cord Blood CD34+CD38- Cell Transduction via Lentivirus-Based Gene Transfer VectorsHuman Gene Therapy, 1999
- No Discrepancy between in Vivo Gene Marking Efficiency Assessed in Peripheral Blood Populations Compared with Bone Marrow Progenitors or CD34+ CellsHuman Gene Therapy, 1999
- Retrovirus-Mediated Gene Transfer into Human CD34+38lowPrimitive Cells Capable of Reconstituting Long-Term CulturesIn Vitroand Nonobese Diabetic–Severe Combined Immunodeficiency MiceIn VivoHuman Gene Therapy, 1998
- An Improved Method for Generating Retroviral Producer Clones for Vectors Lacking a Selectable Marker GeneBlood Cells, Molecules, and Diseases, 1998
- A GFP reporter system to assess gene transfer and expression in human hematopoietic progenitor cellsGene Therapy, 1997
- Comparison of Efficiency of Infection of Human Gene Therapy Target CellsviaFour Different Retroviral ReceptorsHuman Gene Therapy, 1996
- In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral VectorScience, 1996
- Inducible, High-Level Production of Infectious Murine Leukemia Retroviral Vector Particles Pseudotyped with Vesicular Stomatitis Virus G Envelope ProteinHuman Gene Therapy, 1995
- Engraftment of Immune-Deficient Mice with Human Hematopoietic Stem CellsScience, 1988