Comparison of enzyme linked immunosorbent assay and enzyme linked fluorescence immunoassay for detection of antibodies against Chlamydia trachomatis.

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Abstract
An enzyme linked fluorescence immunoassay (ELFA) was evaluated for the detection of antibodies against C. trachomatis. Reticulate bodies and elementary bodies from C. trachomatis L2/434 strain were used as antigens. An enzyme linked immunosorbent assay (ELISA) was also evaluated using the same antigens. Results obtained by ELISA and ELFA for human sera with these 2 antigens were compared with each other and with the results obtained by a micro-immunofluorescence (micro-IF) test. Serum IgG antibodies against C. trachomatis L2 reticulate bodies and elementary bodies were found in 32 (20.0%) and 11 (6.9%), respectively, of 160 serum samples from pregnant women by the micro-IF test (titer .gtoreq. 1/32). All of these 32 pregnant women had IgG antibodies to C. trachomatis reticulate bodies (titer .gtoreq. 1/100), whereas 20 (12.5%) had IgG antibodies to elementary bodies in the ELISA. On the other hand, 25 (15.6%) and 19 (11.9%) of them had IgG antibodies to C. trachomatis L2 reticulate bodies and elementary bodies, respectively, by the ELFA (titer .gtoreq. 1/500).