Uniform Production and Bulk Storage of P388 Murine Lymphoma Cells for Antitumor Assay

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Abstract
Summary P388 mouse lymphoma cells have been produced in quantity with little variability. Difficulties (i.e., O2 deficiencies in spinner flasks and lighted environment for roller bottles) first encountered in the scale-up of a single vial to production volume quantities were overcome. Bulk lots of stored cells proved to be as effective as fresh cells for bioassay, and also provided a rapid method for obtaining production quantities of cells. This reproducible production capability and uniformity, coupled with the flexibility offered by bulk storage provides for a high degree of assay uniformity between and among groups involved in screening antitumor agents.

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