Release of Extracellular Transformable Plasmid DNA from Escherichia coli Cocultivated with Algae
- 1 April 2003
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 69 (4) , 2399-404
- https://doi.org/10.1128/aem.69.4.2399-2404.2003
Abstract
We studied the effects of cocultivation with either Euglena gracilis (Euglenophyta), Microcystis aeruginosa (Cyanophyta), Chlamydomonas neglecta (Chlorophyta), or Carteria inversa (Chlorophyta) on the production of extracellular plasmid DNA by Escherichia coli LE392(pKZ105). Dot blot hybridization analysis showed a significant release of plasmid DNA by cocultivation with all the algae tested. Further analysis by electrotransformation confirmed the release of transformable plasmid DNA by cocultivation with either E . gracilis , M . aeruginosa , or C . inversa . These results suggest algal involvement in bacterial horizontal gene transfer by stimulating the release of transformable DNA into aquatic environments.Keywords
This publication has 49 references indexed in Scilit:
- Small TalkCell, 2002
- Quorum-sensing in Gram-negative bacteriaFEMS Microbiology Reviews, 2001
- Estimation of the fate of dissolved DNA in thermally stratified lake water from the stability of exogenous plasmid DNAAquatic Microbial Ecology, 2001
- Distribution and origin of dissolved DNA in lakes of different trophic statesAquatic Microbial Ecology, 1998
- Determination of dissolved deoxyribonucleic acid concentration in lake waterAquatic Microbial Ecology, 1996
- Bacterial uptake and utilization of dissolved DNAAquatic Microbial Ecology, 1996
- Plasmid transfer to indigenous marine bacterial populations by natural transformationFEMS Microbiology Ecology, 1994
- GENE TRANSFER BETWEEN DISTANTLY RELATED BACTERIAAnnual Review of Genetics, 1991
- Natural transformation of a marineVibrio species by plasmid DNAMicrobial Ecology, 1990
- Extracellular Deoxyribonucleic Acid of Bacteria and a Deoxyribonuclease InhibitorScience, 1956