Purification and characterization of TTFI, a factor that mediates termination of mouse ribosomal DNA transcription.
Open Access
- 1 September 1988
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 8 (9) , 3891-3897
- https://doi.org/10.1128/mcb.8.9.3891
Abstract
Termination of rRNA gene transcription is dependent on an 18-base-pair sequence motif, AGGTCGAC CAG AT TA NTCCG (the Sal box), which is present several times in the spacer region downstream of the 3' end of the pre-rRNA coding region. We report here the purification to molecular homogeneity of a nuclear factor which specifically interacts with the Sal box element. Addition of the isolated protein to S-100 extracts which contain low levels of the Sal box-binding protein and are therefore termination incompetent restores terminating activity, indicating that this protein is a polymerase I-specific transcription termination factor. The purified protein (termed TTFI) has a molecular weight of approximately 105,000 on sodium dodecyl sulfate-polyacrylamide gels. Mild proteolysis generates a relatively protease-resistant core which still specifically recognizes its target sequence. However, the termination activity has been lost, suggesting that the interaction with the DNA and the interaction with the transcription apparatus reside in different protein domains.This publication has 21 references indexed in Scilit:
- Regulation of Inducible and Tissue-Specific Gene ExpressionScience, 1987
- Selective proteolysis defines two DNA binding domains in yeast transcription factor τNature, 1986
- TRANSCRIPTION OF CLONED EUKARYOTIC RIBOSOMAL RNA GENESAnnual Review of Biochemistry, 1986
- TRANSCRIPTION TERMINATION AND THE REGULATION OF GENE EXPRESSIONAnnual Review of Biochemistry, 1986
- A repeated 18 bp sequence motif in the mouse rDNA spacer mediates binding of a nuclear factor and transcription terminationCell, 1986
- Transcription of mouse rDNA terminates downstream of the 3′ end of 28S RNA and involves interaction of factors with repeated sequences in the 3′ spacerCell, 1985
- Domains of the positive transcription factor specific for the Xenopus 5S RNA geneCell, 1984
- Selective and accurate initiation of transcription at the ad2 major late promotor in a soluble system dependent on purified rna polymerase ii and dnaCell, 1979
- Reconstitution of bacterial DNA‐dependent RNA‐polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcriptionFEBS Letters, 1970
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970