Cholesterol Oxidases: A Study of Nature's Approach to Protein Design
- 6 August 2003
- journal article
- review article
- Published by American Chemical Society (ACS) in Accounts of Chemical Research
- Vol. 36 (9) , 713-722
- https://doi.org/10.1021/ar9800587
Abstract
Cholesterol oxidases are important as clinical reagents, potential larvicides, and tools in cell biology, and they are implicated in bacterial pathogenesis. Here we review chemical aspects of their function. We describe our current structural and mechanistic understanding of the type I and II cholesterol oxidases, our identification of an NH−π hydrogen bond motif for stabilization of reduced flavins, our structural hypothesis of how O2 gains access to the flavin, and our present understanding of type I cholesterol oxidase−lipid bilayer interactions.Keywords
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