Luminescence biosensors

Publisher Website
Google Scholar
Abstract
A novel optical biosensor for homogeneous immunoassay has been developed on the basis of the finding that electrochemical luminescence of pyrene‐labelled antigen is extremely inhibited by immunochemical complexation. Electrochemical luminescence homogeneous immunoassay for human serum albumin (HSA), as a model analyte, was performed with a platinum plate electrode which was located in the vicinity of an optical fibre tip. HSA was determined in the concentration range of 3–25 × 10−6 mol/I.To improve electrochemical luminescence measurement an optical fibre electrode has been developed by fabricating a transparent platinum film on the top of an optical fibre. The minimum detectable limit of luminol was 10−11 mol/l with the optical fibre electrode. Luminol was applied as a label for homogeneous immunoassay.

This publication has 4 references indexed in Scilit: