IGE-SELECTIVE AND ANTIGEN-SPECIFIC UNRESPONSIVENESS IN MICE .1. INDUCTION OF THE UNRESPONSIVENESS BY ADMINISTRATION OF OVALBUMIN-PULLULAN CONJUGATE

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 122  (4) , 1266-1272
Abstract
An experimental model was demonstrated in mice for the induction of Ig[immunoglobulin]E-selective unresponsiveness to ovalbumin (OA), a protein antigen [Ag]. An administration of OA conjugated with pullulan, a linear polymer of glucose, (OA-pullulan) into mice resulted in the induction of a long-lasting, IgE-selective unresponsiveness to the subsequent immunization with native OA in the form optimal to elicit IgE antibody [Ab] response. The IgE-selective unresponsiveness is Ag specific and is transferred to normal mice by the spleen cells from mice receiving OA-pullulan conjugate at least 2 wk before. In contrast to other modified Ag, OA-pullulan elicited good IgM and IgG Ab responses, but not an IgE response, without the aid of an adjuvant.