A sensitive spectrofluorimetric method for the determination of human serum albumin with chrome-azurol S.

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Abstract
An extremely sensitive spectrofluorimetric method for assay of human serum albumin (HSA) was developed, based on binding of chrome-azurol S(CAS) by HSA. The CAS-HSA complex shows a fluorescence at 616nm, whereas CAS alone exhibits only a weak fluorescence at this wavelength. A nonionic detergent, polyethylene glycol-p-nonylphenyl ether n=10, acts as a stabilizer of the CAS-HSA complex. The calibration curve was linear in the range from 5 to 80 .mu.g of HSA in the final solution. Relative standard deviation was 1.3% (n=10). The method requires less than one-thirtieth of the amount of HSA required by the control bromcresol green method. HSA values in control sera and spinal fluid assayed by the new method were in agreement with those obtained by the control bromcresol green method.