Cytochemistry of the Normal and X-Irradiated Spermatogenesis of Stenobothrus viridulus (Fischer)

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Abstract
Results of cytochemical observations in the normal and X-irradiated spermatogenesis of Stenobothrus viridulus were as follows. (a) The nucleus of the normal cell contains basic proteins, DNA, and acid mucopolysaccharides containing carboxyl groups only. The nuclear membrane, however, shows the presence of lipids. In the irradiated nucleus, the site of DNA and basic proteins are clearly marked, the former aggregated along the nuclear membrane while the latter concentrated in the center. (b) The mitochondria and the mitochondrial nebenkern of the normal material consists of tyrosine and arginine. The outer membrane of the nebenkern has acidic lipids and cholesterol. In the irradiated material, the intensity of the reactions increased with the coagulation of the proteins. (c) The Golgi bodies consist of galactogen, acid mucopolysaccharides (sulphuric acid groups only), and acidic lipids. In irradiated material, the polysaccha-rides lose their staining power while lipids form the outer membrane. (d) The acrosome is PAS positive and contains tyrosine. It becomes more pronounced in abnormal material. (e) The centriole region consists of lipids plus proteins. The latter coagulate in the irradiated material, and they are clearly differentiated from the lipids.

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