Corneal cell adhesion and proliferation on hydrogel sheets bound with cell-adhesive proteins
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Current Eye Research
- Vol. 10 (10) , 899-908
- https://doi.org/10.3109/02713689109020325
Abstract
The efficiency of protein immobilization to provide a hydrogel surface with a high affinity for corneal epithelial cells was investigated. The cell adhesive proteins collagen and fibronectin, and the cell adhesive oligopeptide RGDS(arginine-glycine-aspartic acid-serine) were successfully covalently immobilized on poly(vinyl alcohol)(PVA) hydrogel sheets. Corneal epithelial cells and L-929 cells were applied to these surfaces. A large number of cells did adhere and proliferate on these protein-bound surfaces in comparison with the virgin PVA not so immobilized.Keywords
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