Composition of the Lipopolysaccharide from Different Capsular Serotype Strains of Haemophilus influenzae
Open Access
- 1 June 1987
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 133 (6) , 1443-1451
- https://doi.org/10.1099/00221287-133-6-1443
Abstract
Summary: Lipopolysaccharide (LPS) from all six serotype strains of Haemophilus influenzae was similar in composition. The oligosaccharide, of each LPS, was composed of glucose, galactose, heptose and 2-keto-3-deoxyoctonic acid. The lipid A was composed of glucosamine, phosphate and the fatty acids 14:0 and 3-OH 14:0. Each LPS also contained ethanolamine and ethanolamine phosphate, and the oligosaccharides from two strains additionally contained small amounts of glucosamine. Although the LPS was similar in composition, different serotypes had quantitative differences, especially in the galactose content, which correlated with the antigenic specificity of their homologous antisera and with their mobility on SDS-polyacrylamide gel electrophoresis (SDS-PAGE). A survey by SDS-PAGE showed that LPS from strains of the serotypes a, c and d was characteristically of lower Mr than the LPS from most (80%) serotype b strains.This publication has 3 references indexed in Scilit:
- Characterization of Lipopolysaccharide of Haemophilus influenzaeThe Journal of Infectious Diseases, 1978
- The morphologic structure of isolated bacterial lipopolysaccharideJournal of Molecular Biology, 1967
- A modified single solution method for the determination of phosphate in natural watersAnalytica Chimica Acta, 1962