Improving membrane voltage measurements using FRET with new fluorescent proteins
- 11 July 2008
- journal article
- Published by Springer Nature in Nature Methods
- Vol. 5 (8) , 683-685
- https://doi.org/10.1038/nmeth.1235
Abstract
We used two new coral fluorescent proteins as fluorescence resonance energy transfer (FRET) donor and acceptor to develop a voltage sensor, named Mermaid, that displays approximately 40% changes in emission ratio per 100 mV, allowing for direct visualization of electrical activities in cultured excitable cells. Notably, Mermaid has fast on-off kinetics at warm (approximately 33 degrees C) temperatures and can report voltage spikes comparable to action potentials.Keywords
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