DOUBLE STAINING BY VAPORIZED OSMIUM-TETROXIDE HYDRAZINE HYDRATE OF BIOLOGICAL SPECIMENS FOR NON-COATED SCANNING ELECTRON-MICROSCOPY

  • 1 January 1982
    • journal article
    • research article
    • p. 459-464
Abstract
Sufficient electron conductivity was obtained in biological specimens following double staining with vaporized OsO4 and hydrazine hydrate for non-gold coated scanning electron microscopic observations. No charging was noted at 27 kV acceleration voltage in rat kidney, liver or other specimens. The foot processes of the glomerular podocytes and micropores of the glomerular endothelium as well as the intracellular endoplasmic reticulum of liver cells were particularly well visualized without the addition of evaporated metal. Repeating this vapor phase osmication method produced even higher contrast at high magnifications compared to the single vaporized osmication method.