Studies on the proteins involved in the interaction of high‐density lipoprotein with isolated human small intestine epithelial cells

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Abstract
Treatment of125I‐labelled high‐density lipoprotein ([125I]HDL3) with monospecific polyclonal antibodies against apolipoproteins A‐I and A‐II resulted in a dose‐dependent inhibition of the [125I]HDL3 binding to isolated human small intestine epithelial cells by 25%, and 50%, respectively. Both antibodies also inhibited intracellular degradation of [125I]HDL3 by 80%. Treatment of enterocytes with polyclonal antibody against apolipoprotein A‐I binding protein, a putative HDL receptor, inhibited both binding and degradation of [125I]HDL3 by these cells by 50%. Antibodies to apolipoprotein A‐I, A‐II and apo A‐I‐binding protein also inhibited [125I]HDL3 binding to cholesterol‐loaded cells.