Membrane Damage to Immunologically Immobilized Rabbit Spermatozoa Visualized by Immunofluorescence and Scanning Electron Microscopy1
Open Access
- 1 September 1975
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 13 (2) , 136-141
- https://doi.org/10.1095/biolreprod13.2.136
Abstract
Rabbit sperm fluoresced relatively uniformly following treatment with goat or rabbit antirabbit semen globulin alone or in combination with heated complement. However, following an immobilizing treatment, namely, fluorescein conjugated goat or rabbit antirabbit semen globulin plus active C′, the sperm fluoresced minimally in the acrosomal region but brightly in the post acrosomal area. This appearance corresponds to the position of the cell membrane of the sperm head following the rolling back of the cell membrane seen by transmission electron microscopy (Russo and Metz, 1974). Comparable posterior fluorescence was observed by indirect immunofluorescence. This posterior fluorescent pattern may be diagnostic for immunologically immobilized rabbit sperm. Membrane damage associated with immunological immobilization was also seen with SEM. This appeared as an accumulation of material interpretable as a “rolling back” of the cell membrane from the acrosomal apex.Keywords
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