Dendritic Cells Induce CD4+ and CD8+ T‐Cell Responses to Mycobacterium bovis and M. avium Antigens in Bacille Calmette Guérin Vaccinated and Nonvaccinated Cattle

Open Access
Publisher Website
Google Scholar
Abstract
Few data are available regarding the induction of memory T‐lymphocyte responses in cattle following Bacille Calmette Guérin (BCG) vaccination. Studies of the immune response induced by BCG vaccination provide an insight into the basis of antimycobacterial immunity that could be exploited for the development of more effective vaccination strategies. We used autologous dendritic cells (DC) infected with Mycobacterium bovis Bacille Calmette Guérin (BCG) or pulsed with purified protein derivative from M. bovis (PPD‐B) or M. avium (PPD‐A) to assess responses of CD4+, CD8+ and WC1+γδ TCR+ lymphocytes from BCG vaccinated and nonvaccinated cattle. Mycobacteria‐specific CD4+ and CD8+, but not WC1+γδ TCR+, memory T lymphocytes were demonstrated in BCG‐vaccinated cattle. CD4+ and CD8+ lymphocytes proliferated and produced interferon (IFN)‐γ in response to BCG‐infected or PPD‐B‐pulsed DC. Proliferative responses were greater for CD4+ than CD8+ lymphocytes, although secretion of IFN‐γ was higher from the CD8+ T cells. Responses to PPD‐A‐pulsed DC were lower, with no CD8+ response. Lymphocytes from nonvaccinated calves were also stimulated to proliferate by BCG‐infected DC, although the magnitude of proliferation was lower. The findings suggest that immunity to M. bovis induced by BCG vaccination in cattle may involve CD8+ memory T cells which produce IFN‐γ, as well as CD4+ memory T cells.

This publication has 0 references indexed in Scilit: