Chemical Fingerprinting of Hoodia Species and Related Genera: Chemical Analysis of Oxypregnane Glycosides Using High-Performance Liquid Chromatography with UV Detection in Hoodia gordonii
Open Access
- 1 November 2007
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 90 (6) , 1526-1531
- https://doi.org/10.1093/jaoac/90.6.1526
Abstract
Hoodia gordonii, family Asclepiadaceae, is a succulent plant and is traditionally used in southern Africa for its appetite-suppressant properties. A high-performance liquid chromatographic (HPLC) method with UV detection for analysis of 11 oxypregnane glycosides from H. gordonii has been developed. The simultaneous analysis of 11 oxypregnane glycosides was achieved with a Phenomenex (Torrance, CA) reversed-phase C18 column using gradient mobile phase of water and acetonitrile, both containing 0.025 trifluoroacetic acid. The developed method was applied to the identification of oxypregnane glycosides in 3 different species of Hoodia and 23 related genera. The HPLC profiles of various plant samples were compared for the presence of oxypregnane glycosides.Keywords
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