The release of (3H)-GABA from the rabbit retina

Publisher Website
Google Scholar
Abstract
The neuronal and glial release of (3H)-GABA from rabbit retina was studied. There are no glutamate, aspartate or glycine receptors on the GABA accumulating neurons, and no GABA autoreceptors. (3H)-GABA was released by 40 mM K+ from retinal neurons, but not from glia, and the release was not dependent on extracellular Ca2+. This indicates a release from a nonvesicular transmitter pool. Ouabain has been proposed as a pharmacological tool for studying the release of (3H)-GABA located in neuronal cytoplasm. It induced an increased release of (3H)-GABA from both neurons and glia and it is therefore unlikely that it can be used for the specific purpose of studying release from neuronal cytoplasm.