Abstract
The observed low-frequency motions with wave numbers of 22 cm-1 and 25 cm-1 for insulin and lysozyme, respectively, originate from the accordion-like motions of the principal helices therein. The calculated results based on such a model are in good agreement with the observed values. During calculations the role of the internal microenvironment on the low-frequency motion is naturally revealed, so as to elucidate as well why this kind of low-frequency motion is so sensitive to the conformation of proteins observed.

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