Studies on the Dna Content, Dry Mass and Optical Area of Morphologically Normal and Abnormal Bull Spermatozoal Heads

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Abstract
Quantitative microspectrophotometric, microinterferometric and microplanimetric techniques were used to compare morphologically normal and abnormal bull spermatozoal heads with respect to DNA content, total dry mass and optical area. Spermatozoa were obtained from ejaculates of two young bulls with disturbances in spermatogenesis. U. v. microspectrophotometric results exhibited no difference in mean amounts of DNA between normal and abnormal spermatozoal heads. Apparently contradictory results were found with Feulgen microspectrophotometry and were discussed. Highly significant differences in mean values were observed between the normal and abnormal heads for total dry mass and optical area. The only cytophotometric parameter in which a significant difference existed between the variances of the two morphological groups was optical area.