'Lens-on-surface' : a versatile method for the investigation of plasma protein exchange reactions on solid surfaces

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Abstract
The exchange sequence of plasma proteins in narrow spaces on solid surfaces was studied by means of a modified 'lens-on-surface' method as originally described by Vroman and Adams [1]. In our studies, lateral scanning ellipsometry was used as the detection method. With the use of antibodies it was demonstrated and confirmed that immunologically detectable plasma protein antigens appear and disappear in a time- and concentration-dependent sequence [IgG followed by fibrinogen followed by high-molecular weight kininogen (HMWK)] on silica surfaces. Plasma protein exchange reactions were also studied on hydrophilic titanium (Ti), vanadium (V), and silver (Ag) surfaces. Atypical exchange patterns were found on V and Ag surfaces as compared with hydrophilic silica (adsorbed fibrinogen was not removed).