Independent rearrangement of Igλ genes in tissue culture-derived murine B cell lines

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Abstract
Rearrangement of the λ light chain locus is considerd a late event In pre-B cell differentiation which occurs after successful heavy chain and unsuccessful χ light chain rearrangement. However, this view has recently been challenged by the observation of apparently independent X rearrangement In certain B cell lines and Ig transgenlc populations. In this study we have examined the pattern of Ig rearrangements and expression in several tissue culture-derived murine Bcell lines. One pre-B cell line (BDL-1) displayed germllne heavy and χ light chain genes despite the presence of a productive λ1 light chain rearrangement. Two other cell lines (DAC-2, BDL-2) had multiple λ rearrangements despite the presence of productive x chain rearrangements. These cell lines provide new precedents for rearrangement of theλ locus independent of the x locus. Their phenotype suggests that accessibility at the different Ig loci may be controlled by a non-sequential mechanism.

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