Étude de la localisation ultrastructurale des activités phosphatasiques acides dans deux types d'Ectomycorhizes:Pinus nigra nigricans/Hebeloma crustuliniformeetPinus pinaster/Pisolithus tinctorius

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Abstract
Ultrastructural localization of acid phosphatases activities was studied in two Basidiomycete ectomycorrhizas grown under controlled conditions. Enzymatic activity was found associated with plasmalemmas, but not with the walls, of living roots cells and fungal hyphae. A strong activity was also detected in the cytoplasm of senescent hyphae in the mantle and in the Hartig net. The strength of the activity in the interhyphal matrix was variable; a strong activity was found in the matrix in the mantle, but only a weak and diffuse activity in the matrix in the Hartlig net. The external surfaces of hyphae at the interface of the mantle and the rooting medium also showed strong phosphatase activity. It is probable that the enzymatic activities located on the plasmalemma of roots cells and fungal hyphae correspond to membrane-bound phosphatases, those detected in the interhyphal matrix represent parietal phosphatases, and those on the surface of the hyphal walls to a combination of excreted phosphatases and phosphatases liberated from senescent hyphae.