IMMUNOLOGICAL STUDIES ON A FREEZE-SUBSTITUTION METHOD OF PREPARING TISSUE FOR FLUORESCENT ANTIBODY STAINING

  • 1 January 1961
    • journal article
    • research article
    • Vol. 4  (3) , 206-+
Abstract
(1) A freeze-substitution, wax embedding method for preparing tissue for fluorescent antibody studies is described. (2) Solutions of ovalbumin and gamma globulin were subjected to the whole embedding procedure and their solubility and immunological reactivity estimated quantitatively. It was found that over 60 per cent of these proteins remained soluble after embedding. The precipitating power of the soluble fraction of ovalbumin and antibody globulin was found to be reduced by half. The insoluble fraction of antibody globulin bound more antigen than would be precipitated at equivalence.

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