In Vitro Propagation of Rose1

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Abstract
Cultured shoot tips and lateral buds from greenhouse-grown rose (Rosa hybrida L. cv. Improved Blaze) proliferated multiple shoots on a basal medium (MS salts, vitamins, glycine, sucrose, and agar) supplemented with 3.0 mg/liter 6-benzylamino purine (BA) and 0.3 mg/liter indoleacetic acid (IAA). A 3-fold multiplication was achieved from freshly explanted terminal shoot tips or lateral buds after 8 weeks. Reculture of in vitro-derived shoots onto the same medium resulted in a 6-fold increase in 8 weeks. Roots could be initiated from about 50% of these shoots after transfer to a medium containing 0.3 mg/liter IAA and 0 or 0.3 mg/liter BA. Regenerated plants were successfully transferred to soil after 2 weeks.

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