The rye (Secale cereale L.) chromosomes of four series of wheat (Triticum aestivum L.) ‐ rye addition line were identified according to their heterochromatic banding patterns using Leishman staining. The characteristics of chromosomes 1R, 3R, 4R/7R, 5R, and 6R were relatively consistent from series to series, while 2R and 7R/4R varied considerably and were therefore difficult to classify. In some of the six triticales (✕ Triticosecale Wittmack) analyzed, certain rye chromosomes easily identified as 2R and 4R/7R, were absent.