Cell Surface Antigens: Serial Sectioning of Single Cells as an Approach to Topographical Analysis
- 30 April 1971
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 172 (3982) , 472-474
- https://doi.org/10.1126/science.172.3982.472
Abstract
The topographical distribution of H-2 antigens on the surfaces of C57BL/6 mouse thymocytes and lymph-node lymphocytes was investigated by a new technique. Single cells were reacted with visually labeled antibody directed against H-2 antigen. Each cell was processed for electron microscopy individually and was serially sectioned. Models constructed from serial electron micrographs provided detailed views of the entire cell surface and showed that H-2 antigen occurs on thymocytes in small isolated regions and on lymphocytes in large interconnected regions.Keywords
This publication has 5 references indexed in Scilit:
- G (Gross) and H-2 Cell-Surface Antigens: Location on Gross Leukemia Cells by Electron Microscopy with Visually Labeled AntibodyProceedings of the National Academy of Sciences, 1970
- ANTIGENIC STRUCTURE OF CELL SURFACESThe Journal of Experimental Medicine, 1969
- New Visual Markers of Antibody for Electron MicroscopyNature, 1969
- USE OF HYBRID ANTIBODY WITH ANTI-γG AND ANTI-FERRITIN SPECIFICITIES IN LOCATING CELL SURFACE ANTIGENS BY ELECTRON MICROSCOPYThe Journal of Experimental Medicine, 1968
- A new method for transferring sections from the liquid surface of the trough through staining solutions to the supporting film of a gridJournal of Ultrastructure Research, 1966