The Use of Incident Light in Immunofluorescence Applied to Syphilis Serology

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Abstract
A method in which the use of incident light was adapted to the automated fluorescent treponemal antibody test has been evaluated. Incident light was superior in many aspects to the conventional transmitted light. Greater intensity of fluorescence, time saving, ease of operation, and economy of reagents were some of the advantages obtained. About 100 slides per 15 min. could be read qualitatively and quantitatively, compared with 15 slides per 15 min. by the conventional method. Agreement between the two methods in the identification of total reactive sera was 100%. The method might be adaptable to sophisticated photometric instruments for automatic measurements.

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