Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis
- 1 December 1982
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 188 (2) , 353-355
- https://doi.org/10.1007/bf00332700
Abstract
The gene coding for the 33,000 dalton protein (B protein) of the mini-F plasmid has been mapped by minicell polypeptide analysis to 47.3–48.2 Fkb. Transcription of the gene is initiated near 47.3 Fkb. Gene B mutants overproduce the 42,000 dalton protein (A protein) of mini-F. Insertions of Tn 5 in the B gene and in the incD region cause instability of plasmid inheritance. Mini-F plasmids delected for part of gene B are not maintained in the absence of the incD region. B protein and the incD region appear to interact in promoting mini-F maintenance.This publication has 15 references indexed in Scilit:
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