Resistance of the Chorio-Allantoic Membrane of Chick Embryos to Rous Sarcoma and MH2 Reticuloendothelioma Viruses

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Abstract
The resistance of chorio-allantoic membranes of various strains (White Leghorn, Australorp, and New Hampshire, from the Kok Poultry Farm; Light Sussex, from the Dept. of Agriculture, Malaya) of chick embryos to Rous sarcoma virus (RSV) and MH2 virus was studied. The New Hampshire, Kok strain was more resistant to RSV and MH2 virus than the other strains (level of resistance was based on the resistant membranes). One strain, White Leghorn, Kok, was more resistant to RSV than to MH2 virus. There was no correlation between resistance of the chorio-allantoic membrane and resistance of the adult chick or the embryo proper to RSV. Experiments with varying titers of RSV and MH2 virus showed that the percentage of nonreactors decreased with increase in virus titer—due to the resistant membranes producing a mild reaction when exposed to higher concentrations of virus. Such resistant membranes with mild reaction should be ignored in the calculation of virus titer from the pock count on membranes. The difference in the mean pock count between different strains of chick embryos to these viruses appeared to be due only to the resistant membranes. The number of pocks produced on the membrane were abnormally distributed and had to be log transformed. Reciprocal crosses between two of the susceptible strains and the resistant New Hampshire, Kok strain show that resistance to RSV and MH2 virus is dominant and not sex-linked.

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