Detection of two different nuclear genomes in parasexual hybrids by ribosomal RNA gene analysis

1 January 1983

journal article
research article
Published by Springer Nature in Theoretical and Applied Genetics

Vol. 64 (2) , 117-118
https://doi.org/10.1007/bf00272717

Abstract

Restriction endonucleases discriminated between the rDNAs contained in callus tissues derived from Nicotiana glauca, N. langsdorffii, and their somatic hybrids produced by protoplast fusion. With XbaI, a single repeat fragment of 7.5 × 10⁶ daltons was produced from N. glauca rDNA compared to a single repeat fragment of 4.2 × 10⁶ daltons produced from N. langsdorffii rDNA. Both kinds of XbaI fragments were found in somatic hybrids.

Keywords

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