A comparison of the effect of four benchmark chemicals onDaphnia magnaandCeriodaphnia dubia‐affinistested at two different temperatures

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Abstract
The purpose of this study was to compare the sensitivity ofCeriodaphnia dubia/affiniswith that ofDaphnia magnato chlorobenzene, phenol, diethanolamine and ethylene glycol at 20 and 24°C. Static acute toxicity tests (48‐h) were carried out at these temperatures in environmental chambers devoted only to toxicity studies. The majority of toxicity tests were carried out non‐concurrently, in triplicate, and in each triplicate series the concentrations were randomly assigned within the appropriate ranges.Response differences of the two organisms at the two test temperatures to the four benchmark chemicals are of the same order of magnitude in each case.D. magnaexhibited no statistically significant difference in its response to phenol, diethanolamine and ethylene glycol at the two test temperatures. However,D. magnais less sensitive to chlorobenzene at 24°C than at 20°C.C. dubia/affinisis more sensitive to phenol, diethanolamine and ethylene glycol at 24°C than at 20°C. LikeD. magna, C. dubia/affinisis less sensitive to chlorobenzene at 24°C than at 20°C, but the difference for the latter is not statistically significant.A comparison of the two organisms shows thatC. dubia/affinisis more sensitive to the four benchmark chemicals at 24°C than at 20°C. At 20°C, C.dubia/affinisis more sensitive to chlorobenzene and ethylene glycol than isD. magna, but the response to phenol and diethanolamine is the same for both organisms.