Measurement of Large Distances in Biomolecules Using Double-Quantum Filtered Refocused Electron Spin−Echoes
- 1 June 2004
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (25) , 7746-7747
- https://doi.org/10.1021/ja049372o
Abstract
It is shown how the new technique of double-quantum filtered refocused electron spin-echoes is a significant improvement over double-quantum coherence ESR, since it increases the experimental acquisition time. This enables the measurement of longer distances in bilabeled biomolecules. The method is demonstrated on a long double-stranded A-type RNA, spin labeled at both ends. The measured distance of 72 A is in excellent agreement with molecular modeling.Keywords
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