Somatic hybridization of two selected single cells
- 1 December 1983
- journal article
- Published by Wiley in Cell Biology International Reports
- Vol. 7 (12) , 1123-1128
- https://doi.org/10.1016/0309-1651(83)90019-x
Abstract
Two single mesophyll-protoplasts of Nicotiana tabacum cv. xanthi were selected into a 100 nl microdroplet of 0.4 M mannitol. Two cylindrical platinum electrodes were inserted into the microdroplet to align the two single cells via dielectrophoresis in an AC-field (1 MHz, about 120 V × cm −1 ). A single square DC-pulse of about 1.5 KV × cm −1 was applied to induce protoplast fusion.Keywords
This publication has 0 references indexed in Scilit: