The effects of two disulphides on cholinesterase activity in the spectrophotometric assay
- 1 April 1974
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 139 (1) , 123-127
- https://doi.org/10.1042/bj1390123
Abstract
1. 5,5′-Dithiobis-(2-nitrobenzoate) did not influence serum cholinesterase activity, whereas 2,2′-dithiodipyridine had an inhibitory effect. 2. The lowering of the molar extinction coefficients observed in the presence of physostigmine may be a result of a reaction between thiolate ions with carbamate moieties. 3. The use of 5,5′-dithiobis-(2-nitrobenzoate) is still recommended in investigations, especially where the quantitative aspects are significant.Keywords
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