Lincomycin Resistance Mutations in Two Regions Immediately Downstream of the −10 Region of lmr Promoter Cause Overexpression of a Putative Multidrug Efflux Pump in Bacillus subtilis Mutants
Open Access
- 1 January 2003
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 47 (1) , 432-435
- https://doi.org/10.1128/aac.47.1.432-435.2003
Abstract
We isolated 19 lincomycin-resistant Bacillus subtilis mutants by expressing lmrB encoding a putative multidrug efflux protein. Eighteen of the mutants altered at two regions (−3 to −1 and +15) immediately downstream of the −10 region of the lmr promoter increased lmr transcription in vivo and in vitro.Keywords
This publication has 10 references indexed in Scilit:
- A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiportPublished by Elsevier ,2002
- Efflux-mediated drug resistance in Gram-positive bacteriaCurrent Opinion in Microbiology, 2001
- Expression of the ftsY gene, encoding a homologue of the α subunit of mammalian signal recognition particle receptor, is controlled by different promoters in vegetative and sporulating cells of Bacillus subtilisMicrobiology, 2000
- Promoter Selectivity of the Bacillus subtilis RNA Polymerase A and H HoloenzymesThe Journal of Biochemistry, 1998
- Evolutionary origins of multidrug and drug-specific efflux pumps in bacteriaThe FASEB Journal, 1998
- A 32 kb nucleotide sequence from the region of the lincomycin-resistance gene (22°-25°) of the Bacillus subtilis chromosome and identification of the site of the lin-2 mutationMicrobiology, 1997
- Residues important for the function of a multihelical DNA binding domain in the new transcription factor family of Cam and Tet repressorsProtein Engineering, Design and Selection, 1995
- EmrR is a negative regulator of the Escherichia coli multidrug resistance pump EmrABJournal of Bacteriology, 1995
- A protein that activates expression of a multidrug efflux transporter upon binding the transporter substrates.Journal of Biological Chemistry, 1994
- Membrane transport proteins: implications of sequence comparisonsCurrent Opinion in Cell Biology, 1992