INVESTIGATIONS OF THE AMINO-ACID AND CARBOHYDRATE-METABOLISM ON PORCINE HEMOPHILUS STRAINS BY MEANS OF THIN-LAYER CHROMATOGRAPHY

  • 1 March 1988
    • journal article
    • research article
    • Vol. 267  (4) , 495-505
Abstract
The aim of the present study was to investigate porcine reference and filed strains of the species Haemophilus (H.) pleuropneumoniae and Hemophilus parasuis, as well as H. Taxon "minor group" and Taxon C on their amino acid and carbohydrate metabolism by thin layer chromatography. The 17 reference strains studied showed almost identical results within the different species and taxa in both, amino acid and carbohydrate metabolism patterns. Based on a few differing enzymatic reactions a reduced species- and taxon-specific reaction pattern could be established, which included L-alanine L-citrulline, and L-threonine of the amino acids as well as D-ribose, .alpha.-D-xylose, mannitol, trehalose, .beta.-melibiose and .alpha.-lactose of the carbohydrates. This differentiation system allowed a reliable identification of 7 field strains whereas 4 additional ones, hitherto pre-classified as H. parasuis, could not be associated with any of the above species and taxa.