Effect of sodium and potassium concentrations and pH on the maintenance of motility of rabbit and rat epididymal spermatozoa

Abstract
Spermatozoa were collected from the caput and cauda epididymidis of rabbits and rats and diluted in Hank''s solution containing bovine serum albumin, with various concentrations of Na+ and K+. Ionic strength and osmolarity were kept constant. Motility was assessed at various intervals during incubation at 25.degree. C. In the pH range 7.05-7.20, the motility of rabbit spermatozoa was not affected by changes in the ratio of K+ to Na+. The motility of rat cauda spermatozoa was not altered but caput spermatozoa was slightly depressed by a high K+/Na+ ratio. In the pH range 5.45-5.85, rabbit cauda and caput spermatozoa had much greater motility in media with a high K+/Na+ ratio. The reverse result was obtained for the rat. The motility of epididymal spermatozoa is evidently influenced by external Na+ and K+ concentrations. This phenomenon is pH-dependent.

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