Production of poly‐β‐hydroxybutyrate by Azotobacter chroococcum H23 in chemically defined medium and alpechin medium

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Abstract
M.V. MARTINEZ‐TOLEDO, J. GONZALEZ‐LOPEZ, B. RODELAS, C. POZO AND V. SALMERON. 1995. Azotobacter chroococcum H23 is able to produce large amounts of poly‐β‐hydroxybutyrate (PHB) during growth in chemically‐defined medium (N‐free or with NH+4) and alpechin (wastewater from olive oil mills) medium. Polymer production was not dependent of the nutrient limitation. Strain H23 was capable of accumulating PHB up to 70% of the cell dry weight after 24 h incubation in chemically‐defined media containing 1% glucose, fructose, mannitol, saccharose or starch. Azotobacter chroococcum grown on NH+4‐medium supplemented with alpechin formed PHB up to 50% of the cell dry weight after 24 h, suggesting that these wastes could be utilized by Azotobacter as a cheap substrate for producing PHB.