FLOW CYTOFLUOROMETRIC ANALYSIS OF THE EFFECTS OF NEW NITROSOUREA DERIVATIVES ON PROLIFERATION OF EMT-6 TUMOR-CELLS INVITRO

  • 1 January 1981
    • journal article
    • research article
    • Vol. 35  (1) , 27-29
Abstract
Examined by flow cytofluorometric analysis, the DNA distribution of EMT 6 [mouse mammary] 6 tumor cells was highly pertubed after 1 h of in vitro incubation with RPCNU [(chloro-2-ethyl)-1-(ribopyranosyl-triacetate-2''-3''-4'')-3-nitrosourea], RFCNU [(chloro-2-ethyl)-1-(ribofuranosylisopropylidene)-2''-3''-paranitrobenzoate-5''-3-nitrosourea], chlorozotocin (CZT) or I 85 (CNCC [(di-chloro-ethyl-nitrosocarbamoyl)-cystamine]), 4 new nitrosourea derivatives. After treatment with chlorozotocin (20 .mu.g/ml) and CNCC (50 .mu.g/ml), most cells were in G2 + M phase; this accumulation lasted more than 48 h without any restoration before 72 h RPCNU (20 .mu.g/ml) and RFCNU (50 and 65 .mu.g/ml) induced an accumulation of cells in G2 + M phase during 24 h; the normal state was regained after 48 h. These reduced rates of progression of the cells through S phase and the G2 block observed after exposure to the new compounds, should, in part, explain their antitumor activity.