THE EFFECT OF HIGH PRESSURE TREATMENT ON THE PHYSIOLOGICAL ACTIVITY OF INSULIN

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Abstract
Insulin solns. made from Lilly''s Iletin U-20 and from powdered amorphous material to strength of 2.5 phys. units per c.c. of soln. were subjected to high pressure treatment (hydrostatic) as great as 10,000 kg/cm2 for 41 hrs. at room temp. without change of activity as measured by the change of blood sugar level in rabbits. Denaturation and coagulation occurred but no change of Van Slyke amino N was observed. The behavior of insulin in respect to other proteins under these conditions is discussed and it is suggested that denaturation by pressure is fundamentally different, from the energy standpoint, from denaturation by heat that is known to reactivate insulin.

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