Immobilization ofAspergillus FicuumPhktase: Product Characterization of this Bioreactor
- 1 December 1988
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 18 (4) , 483-489
- https://doi.org/10.1080/00327488808062546
Abstract
Aspergillus ficuum phytase was covalently immobilized on Fractogel TSK HW-75 containing 2-oxy-l-alkylpyridinium salts. A packed-bed bioreactor was constructed with the immobilized phytase. An HPLC ion-exchange method was used to analyze the enzymatic products of the bioreactor. Immobilized fungal phytase was able to hydrolyze myo-lnositol Hexa-, penta-, tetra-, tri-, and diphosphates. When the substrate solution was recirculated for 5 hr in the bioreactor about 50% inorganic orthophosphate was released and myoinositol-diphosphate and mono-phosphate were the only remaining products.This publication has 14 references indexed in Scilit:
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