Peptidoglycan Transpeptidase Inhibition in Pseudomonas aeruginosa and Escherichia coli by Penicillins and Cephalosporins
Open Access
- 1 April 1979
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 15 (4) , 513-517
- https://doi.org/10.1128/aac.15.4.513
Abstract
Peptidoglycan transpeptidase activity has been studied in cells of Escherichia coli 146 and Pseudomonas aeruginosa 56 made permeable to exogenous, nucleotide-sugar peptidoglycan precursors by ether treatment. Transpeptidase activity was inhibited, in both organisms, by a range of penicillins and cephalosporins, the Pseudomonas enzyme being more sensitive to inhibition in each case. Conversely, growth of E. coli 146 was more susceptible to these antibiotics than growth of P. aeruginosa 56. Furthermore, similar transpeptidase inhibition values were ob-obtained for the four penicillins examined against the Pseudomonas enzyme, although only two of these (carbenicillin and pirbenicillin) inhibited the growth of this organism. We therefore conclude that the high resistance of P. aeruginosa 56 to growth inhibition by most β-lactam antibiotics cannot be due to an insensitive peptidoglycan transpeptidase.This publication has 20 references indexed in Scilit:
- The β-lactamases of Gram-negative bacteria and their rôle in resistance to β-lactam antibioticsJournal of Antimicrobial Chemotherapy, 1976
- Peptidoglycan biosynthesis in a thermosensitive division mutant of Escherichia coliBiochemistry, 1976
- Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes.1974
- THE INTERPLAY OF β‐LACTAMASES AND INTRINSIC FACTORS IN THE RESISTANCE OF GRAM‐ NEGATIVE BACTERIA TO PENICILLINS AND CEPHALOSPORINS*Annals of the New York Academy of Sciences, 1974
- PENICILLIN‐SENSITIVE ENZYMES AND PENICILLIN‐BINDING COMPONENTS IN BACTERIAL CELLS*Annals of the New York Academy of Sciences, 1974
- dd -Carboxypeptidase-Transpeptidase and Killing Site of β-Lactam Antibiotics in Streptomyces Strains R39, R61, and K11Antimicrobial Agents and Chemotherapy, 1973
- Role of the Penicillin-Sensitive Transpeptidation Reaction in Attachment of Newly Synthesized Peptidoglycan to Cell Walls of Micrococcus luteusProceedings of the National Academy of Sciences, 1972
- Penicillin-sensitive transpeptidation during peptidoglycan biosynthesis in cell-free preparations from Bacillus megaterium. II. Effect of penicillins and cephalosporins on bacterial growth and in vitro transpeptidation.1972
- A simple method for following the fate of alanine-containing components in murein synthesis inEscherichia coliAntonie van Leeuwenhoek, 1971
- BIOSYNTHESIS OF PEPTIDOGLYCAN OF BACTERIAL CELL WALLS .13. PEPTIDOGLYCAN TRANSPEPTIDASE AND D-ALANINE CARBOXYPEPTIDASE - PENICILLIN-SENSITIVE ENZYMATIC REACTION IN STRAINS OF ESCHERICHIA COLI1968