Carotenoid stoichiometry in the LH2 crystal: No spectral evidence for the presence of the second molecule in the α/β‐apoprotein dimer
- 15 June 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (16) , 3841-3844
- https://doi.org/10.1016/j.febslet.2006.06.005
Abstract
In this work we have investigated the carotenoid–protein interactions in LH2 complexes ofRhodopseudomonas acidophilaboth in “free in solution” mixed‐micelles and in three‐dimensional crystals by Raman spectroscopy in resonance with the carotenoid (Car) molecules. We show that the Car molecules when bound to their binding pockets show no significant differences when the complexes are “free in solution” or packed in crystalline arrays. Furthermore, there is no significant wavelength dependence in the Raman spectrum of the Car molecules of LH2. This indicates that there is only one Car configuration in LH2 and thus only one molecule per α/β‐heterodimer.Keywords
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