Large Scale Preparation of Bovine Renal Glomerular Basement Membrane in the Presence of Protease Inhibitors
- 31 December 1977
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 8 (2-3) , 215-224
- https://doi.org/10.1080/00327487808069061
Abstract
A method is described for the isolation of large quantities of basement membrane from bovine renal glomeruli under conditions which minimize or prevent degradation by tissue-associated proteases. The method incorporates the use of moderately-high concentrations of the protease inhibitors: ethylene-diamine tetraacetic acid, ε-amino caproic acid, N-ethylmaieimide, and diiso-propylfluorophosphate; and the maintenance of a temperature of 0-4°C throughout the procedure. Glomeruli preparations are isolated by a modified sieving technique and are routinely of purity greater than 97%. Under optimum conditions, three to four grams of basement membrane can be isolated under safe and rapid conditions in one week.This publication has 14 references indexed in Scilit:
- Postproline cleaving enzyme: identification as serine protease using active site specific inhibitorsBiochemistry, 1977
- Platelet Interaction with Collagen Fibrils in Flowing BloodThrombosis and Haemostasis, 1977
- Bovine renal glomerular basement membrane. Assessment of proteolysis during isolationBiochemical and Biophysical Research Communications, 1976
- The primary glomerular filtration barrier—basement membrane or epithelial slits?Kidney International, 1975
- Glomerular Permeability to Protein Molecules – its Possible Structural BasisNephron, 1974
- Studies on the Native and Reduced Alkylated Renal Glomerular Basement MembranePublished by Elsevier ,1972
- Studies on the Renal Glomerular Basement MembranePublished by Elsevier ,1967