Electrophoretic transport of solutes in aqueous two-phase systems
- 1 January 1990
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 11 (8) , 605-611
- https://doi.org/10.1002/elps.1150110804
Abstract
Electrophoretic transport of proteins across the interface between the phases of an aqueous polymer two-phase system can be greatly impeded in comparison with transport within the individual phase. This effect can be controlled by modifying the affinity of the protein for a phase by suitable manipulations of such variables as pH. The effect is not caused by differences in the electrophoretic velocity between the two phases, nor by large changes in pH at the interface. An analogy exists between this phenomenon and the related subject of diffusion of electrolytes across the phase interface.This publication has 17 references indexed in Scilit:
- Theoretical Aspects of PartitioningPublished by Elsevier ,1985
- Detection of surface differences between two closely related cell populations by partitioning isotopically labeled mixed cell populations in two-polymer aqueous phasesCell Biochemistry and Biophysics, 1983
- DNA fractionation by two-phase partition with aid of a base-specific macroligandAnalytical Biochemistry, 1981
- A rapid method for the isolation of circular DNA using an aqueous two-phase partition systemNucleic Acids Research, 1978
- Separation of Erythromycin-Resistant and -Susceptible Subpopulations of Escherichia coli 15 by Partition in Two-Polymer Aqueous PhasesAntimicrobial Agents and Chemotherapy, 1974
- Preparation of Impermeable Inside-Out and Right-Side-Out Vesicles from Erythrocyte MembranesPublished by Springer Nature ,1974
- Diffusion of proteins across a liquid-liquid interfaceBiochimica et Biophysica Acta (BBA) - General Subjects, 1973
- Partition of closely related proteins in aqueous two-polymer phase systems. Human hemoglobin variants and hemoglobins from different speciesBiochemistry, 1971
- A new method for the isolation of deoxyribonucleic acid from microorganismsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- Partition of Proteins in Liquid Polymer–Polymer Two-Phase SystemsNature, 1958