Origin of Migrating Cells in Cultures of Moth Ovarian Tissue Based on Developmental Stages Producing Optimum Outgrowth

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Abstract
Minced ovaries from several developmental stages of Bombyx mori (L.), Galleria mellonella (L.), Estigmene acrea (Drury), Spodoptera frugiperda (J. E. Smith), and Trichoplusia ni (Hübner) were grown in Grace's medium containing 4.8% silkworm hemolymph. Evaluation of the cultures was based on the number of cells produced, the quality of the cells, and the length of life of the cultures. The extent of ovarian development was based on the first appearance of yolk in the ovum, development of follicles in 90% of the length of the ovariole, beginning of contractions in the ovariole sheath, and beginning of degeneration of the intermediate-layer cells. The results indicated that the selection of material for ovarian cultures should be based on a compromise among these factors: large ovary size, large number of immature follicles, and healthy intermediate-layer cells, and, in some species, undifferentiated ovariole sheath muscle. Comparison of the quality of the cultures with the developmental stage of the ovaries indicated that the intermediate layer and the follicular epithelium produced most of the cells in the cultures.

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